微生物反向电渗析反应器产过氧化氢的研究

微生物反向电渗析(Microbial reverse-electrodialysis electrolysis cell, MREC)是一种通过微生物产生的电能与浓淡水盐差能耦合形成的一种新型的生物电化学技术.本文对MREC阴极回收H_2O_2的运行条件与影响因素及同步产能效应进行了研究.结果表明,浓淡水流速及浓度比是影响H_2O_2产生的关键因素.在浓淡水流速为2 mL·min~(-1)、浓/淡水比为100时,MREC可以获得最大H_2O_2产量711.4 mg·L~(-1),产H_2O_2速率达到最大33.65 mg·L~(-1)·h~(-1),阴极回收率为19.77%.同时,对应的产电性能达到1.25 W·m~(-2).MREC反应器能够在无需施加外界能源的情况下获得较高的H_2O_2产量,为H_2O_2绿色生产提供了一条新思路.     

给水管网多相界面中微生物表面疏水性研究

采用疾病控制中心（CDC）生物膜反应器模拟给水管网系统,选取聚氯乙烯（PVC）和聚碳酸酯（PC）2种材质的挂片,通过微生物粘附碳氢化合物（MATH）实验和Illumina高通量测序相结合的方法,对反应器水相、生物膜相和颗粒物相中微生物的疏水性进行了研究.结果显示,PVC材质挂片反应器中优势菌为厚壁菌门,相对丰度为68.31%~81.00%,PC材质挂片反应器中优势菌为变形菌门,相对丰度为24.39%~64.40%.PVC材质挂片反应器中优势菌包含3类致病菌,PC材质挂片反应器中包含8类致病菌.PC材质挂片生物膜相较于PVC材质疏水性更高,利于微生物吸附形成生物膜,而PVC材质不易形成生物膜,对控制输送过程中的二次污染具有积极作用,但在管网实际应用中还应考虑其他工程因素的影响.     

Geographic Variation in the Seasonal Reproductive Strategy of the Mite Varroa jacobsoni in the Honeybee Nest

Variation in the reproduction of Varroa jacobsoni mite was studied in relation to the expansion of the range of its parasitism on the honeybee. Geographic differences in the seasonal dynamics of mite reproduction in the nests of bee families were revealed. Variation in the sex ratio of mites and the factors inhibiting their reproduction at the northern boundary of the honeybee range are considered. The forms of parthenogenetic reproduction in V. jacobsoni are discussed.     

Aseptically collected calf serum as an effective alternative to fetal calf serum in the culture of amniotic fluid cells

The growth-promoting activities of three different bovine sera have been compared in primary and secondary amniotic fluid cell cultures. In secondary amniotic fluid cell micro- cultures, aseptically collected calf serum (CS) was slightly, though not significantly more effective than fetal calf serum (FCS) in promoting DNA synthesis, while newborn calf serum (NCS) was significantly less effective than either CS or FCS. All three sera were optimally effective at concentrations of 10 per cent (v/v). Significant variation in quality occurred within four batches of each of CS and FCS, but not within four batches of NCS. A selected batch of CS was significantly more effective in promoting the growth of primary amniotic fluid cell cultures than were a number of batches of FCS then in routine laboratory use. It is suggested that CS may serve as an effective and economical alternative to FCS in the culture of amniotic fluid cells, thereby expanding the scope of serum batch testing. A possible explanation for the varying growth-promoting activities of different sera is discussed.     

Unexpected structural chromosome rearrangements in prenatal diagnosis

Among 5315 prenatal diagnoses performed for various indications (maternal age, neural tube defect, metabolic diseases, X-linked diseases, pathologic pregnancies) 29 unexpected structural chromosome rearrangements were found in fetal cells. Fourteen were de novo chromosome rearrangements, six unbalanced, and eight balanced. Fifteen were inherited and balanced rearrangements. This high frequency of structural anomalies is discussed.     

Rare cell isolation using antibodies covalently linked to slides: application to fetal cells in maternal blood

We describe here a new type of method for isolation of rare cell populations in biological fluids. The method is based on the anthraquinone technology for covalent binding of molecules to a polymer surface. An anthraquinone molecule conjugated via a linker to an electrophilic group (AQ Immobilizer™ reagent, Exiqon A/S) is covalently bound to a polymer surface by UV irradiation. The electrophilic group of this AQ reagent can covalently bind a specific antibody directed against a specific cell marker. Applying a cell sample to the functional surface, the cells having the specific cell marker on the cell surface will bind to the antibody on the functional surface. Using this technique, even extremely small cell populations may be isolated. We succeeded in isolating fetal cells from maternal blood samples in the first trimester for chromosome defects genetic diagnosis. Copyright © 2003 John Wiley & Sons, Ltd.     

Promotion of hexadecyltrimethyleamine bromide to the damage of Alexandrium sp. LC3 by cupric glutamate

The effect of hexadecyltrimethyleamine bromide （HDTMAB） on the removal of A lexandrium sp. LC3 under cupric glutamate stress was investigated. Toxic effect of cupric glutamate on A lexandrium sp. LC3 was significantly promoted in the presence of HDTMAB, especially at 3.0 cmc of HDTMAB. It was found that the sulfhydryl group content of the cell decreased, while the malonaldehyde content and membrane permeability increased when A lexandrium sp. LC3 was treated with HDTMAB and cupric glutamate complex, compared with cupric glutamate alone. The data suggest that HDTMAB might stimulate the damage of A lexandrium sp. LC3 by enhancing the membrane permeability.     

电极构型对空气阴极生物燃料电池发电性能的影响

在空气阴极生物燃料电池(ACMFC)中,从阴极扩散进入阳极的氧气能够被兼性微生物作为电子受体还原,进而导致电子损失严重.本研究利用葡萄糖作底物,对2种不同电极构型的空气阴极生物燃料电池ACMFC1和ACMFC2的功率输出和电子回收进行了比较研究.结果表明,ACMFC1的内阻为302.14Ω,阳极电位为-323mV,最大功率密度为3 070 mW/m³;ACMFC2的内阻为107.79Ω,阳极电位为-442mV,最大功率密度达到9 800 mW/m³.在间歇条件下,ACMFC2可以连续运行220h,电子回收率为30.1%;而ACMFC1只能运行不到50h,电子回收率为9.78%.因此,合理的设计空气阴极生物燃料电池电极构型可以减小内阻,增大电池电动势进而增大功率输出,提高电子回收率.     

聚氨酯泡沫固定化产碱杆菌细胞生物转化氰化物

利用1株产碱杆菌DN25作为降氰菌株,以聚氨酯泡沫为载体进行固定化,研究其转化特性.结果表明,采用吸附生长法能有效实现菌株DN25的固定,固定细胞量可达到每g泡沫载体生物量干重0.35g.固定化细胞的最适转化温度和pH为35℃、8.0.对于低浓度氰化物,固定化细胞和游离细胞的转化速率相当;对于高浓度氰化物,固定化细胞具有明显优势,不仅可耐受更高浓度的氰化物转化,其转化速率也高于游离细胞,最大转化速率为507mg/(L·h),是游离细胞的2.8倍.通过初步的摇瓶模拟序列批式反应,固定化细胞活性可保持20d.